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1- set up why humidity and osmolarity could be a problem when performing in vitro culture, introducing the negative effects of osmolarity and humidity on embryos growth during incubation In Vitro fertilisation.
2- Definition of the Humdish and introduce the Humdish as a potential solution for these problems
For experiment 1, a simple linear regression will be performed on calculated mean values to test the relationship between oil levels and osmolarity readings.
In experiment 2, logistic regression will be used to look at the relationship between mean osmolarity readings and blastocyst development (as binary data), and an independent T-test will be used to compare dish type and mean osmolarity readings. All data will be tested for normality using a Shapiro-Wilk test and a P-value <0.05 will be considered statistically significant.
1- Clarify significance of results in relation to project aim/s.
2- Suggest reasons for unexpected results.
3- Compare my results to another study with mention the differences and the significant results.
4- acknowledge limitations and suggest remedies (where applicable) by comparing with different studies and experiments